H2N2V1, Main, Exploration, bibRecord, 002974

Temperature-sensitive mutants of influenza virus XV. The genetic and biological characterization of a recombinant influenza virus containing two is lesions produced by mating two complementing, single lesion is mutants

Identifieur interne : 002974 ( Main/Exploration ); précédent : 002973; suivant : 002975

Temperature-sensitive mutants of influenza virus XV. The genetic and biological characterization of a recombinant influenza virus containing two is lesions produced by mating two complementing, single lesion is mutants

Auteurs : Brian R. Murphy [États-Unis] ; Frank T. Wood [États-Unis] ; Judith G. Massicot [États-Unis] ; Susan B. Spring [États-Unis] ; Robert M. Chanock [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1978.

RBID : ISTEX:3D3D58C192949017144E498DC317B139364D00D2

English descriptors

Teeft :
- Attenuated, Attenuation, Biological characterization, Chanock, Clone, Complementation, Complementation group, Complementation groups, Crna, Crna synthesis, Donor virus, Genetic stability, Hamster, Hemagglutinin, Hong virus, Influenza, Influenza virus, Inoculated, Lesion, Mdck, Mdck monolayer cultures, Monolayer, Mutant, Mutation, Nasal, Nasal turbinates, Neuraminidase, Parental virus, Permissive temperature, Plaque, Plaque formation, Plaque titer, Plaque titration, Predictable level, Progeny, Prototype, Prototype viruses, Recombinant, Recombinant clones, Recombinant virus, Recombinant viruses, Recombination, Recombination index, Replication, Restrictive temperature, Richman, Seronegative, Seronegative individuals, Shutoff, Shutoff temperature, Similar restriction, Single infections, Subtotal subtotal, Titer, Turbinate, Unpublished observations, Vaccine, Viral, Virology, Virus, Virus challenge, Virus replication, Wild type.

Abstract

Abstract: The influenza A/Hong Kong/68-ts-1[E] virus and its recombinants (38° shutoff temperature for plaque formation) were satisfactorily attenuated for individuals with neuraminidase immunity who lacked serum antibody for hemagglutinin antigen but not for persons who lacked immunity to both viral surface antigens, i.e., doubly seronegative individuals. It was necessary to produce a virus more defective than the Hong Kong/68-ts-1[E] virus to meet the need for an attenuated virus suitable for use in doubly seronegative persons. We produced a new recombinant which contained a ts mutation(s) on the genes believed to code for the Pl and P3 viral proteins, both of which are involved in cRNA synthesis. This recombinant was produced by mating two complementing ts mutants, one with a mutation affecting the Pl protein and the other with a mutation affecting the P3 protein. The new double ts recombinant, the Udorn/72-ts-1A2 virus, was 700-fold more restricted in plaque formation at 37° than either of its ts parents. In addition, it was defective in complementation-recombination when tested against prototypes of seven complementation groups. The Udorn/72-ts-1A2 recombinant failed to replicate in the hamster's lungs (37°) and was 100-fold restricted in its growth in the nasal turbinates (32°). Each of the isolates from Udorn/72-ts-1A2 infected hamsters was ts. Despite restricted replication in vivo, infection of hamsters with this virus induced significant resistance to wild-type virus challenge. Thus, the Udorn/72-ts-1A2 recombinant was (1) more restricted in replication in vitro at 37° and in vivo in the hamster and (2) more stable genetically in vivo than the HK/68-ts-1[E] virus.

Url:

DOI: 10.1016/0042-6822(78)90280-5

Affiliations:

États-Unis

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<term>Prototype viruses</term>
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<term>Seronegative individuals</term>
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<term>Shutoff temperature</term>
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<term>Single infections</term>
<term>Subtotal subtotal</term>
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<front><div type="abstract" xml:lang="en">Abstract: The influenza A/Hong Kong/68-ts-1[E] virus and its recombinants (38° shutoff temperature for plaque formation) were satisfactorily attenuated for individuals with neuraminidase immunity who lacked serum antibody for hemagglutinin antigen but not for persons who lacked immunity to both viral surface antigens, i.e., doubly seronegative individuals. It was necessary to produce a virus more defective than the Hong Kong/68-ts-1[E] virus to meet the need for an attenuated virus suitable for use in doubly seronegative persons. We produced a new recombinant which contained a ts mutation(s) on the genes believed to code for the Pl and P3 viral proteins, both of which are involved in cRNA synthesis. This recombinant was produced by mating two complementing ts mutants, one with a mutation affecting the Pl protein and the other with a mutation affecting the P3 protein. The new double ts recombinant, the Udorn/72-ts-1A2 virus, was 700-fold more restricted in plaque formation at 37° than either of its ts parents. In addition, it was defective in complementation-recombination when tested against prototypes of seven complementation groups. The Udorn/72-ts-1A2 recombinant failed to replicate in the hamster's lungs (37°) and was 100-fold restricted in its growth in the nasal turbinates (32°). Each of the isolates from Udorn/72-ts-1A2 infected hamsters was ts. Despite restricted replication in vivo, infection of hamsters with this virus induced significant resistance to wild-type virus challenge. Thus, the Udorn/72-ts-1A2 recombinant was (1) more restricted in replication in vitro at 37° and in vivo in the hamster and (2) more stable genetically in vivo than the HK/68-ts-1[E] virus.</div>
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Temperature-sensitive mutants of influenza virus XV. The genetic and biological characterization of a recombinant influenza virus containing two is lesions produced by mating two complementing, single lesion is mutants

Temperature-sensitive mutants of influenza virus XV. The genetic and biological characterization of a recombinant influenza virus containing two is lesions produced by mating two complementing, single lesion is mutants

Source :

English descriptors

Abstract

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